2A3Q

X-Ray Structure of Protein from Mus Musculus MM.29898

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.96802, 0.96411
Spacegroup name	P 65 2 2
Unit cell lengths	73.541, 73.541, 236.078
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.270 - 2.320
R-factor	0.212
Rwork	0.210
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.834
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.350	2.350
Rmerge	0.061	0.446
Number of reflections	17029
<I/σ(I)>	16.785	5.272
Completeness [%]	98.5	100
Redundancy	13.2	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	277	10 MG/ML PROTEIN, 18% MEPEG 2K, 0.100 M SODIUM CITRATE, 0.100 M SODIUM ACETATE, vapor diffusion, hanging drop, temperature 277K, pH 5.00
1		5	277	10 MG/ML PROTEIN, 18% MEPEG 2K, 0.100 M SODIUM CITRATE, 0.100 M SODIUM ACETATE, vapor diffusion, hanging drop, temperature 277K, pH 5.00