2A3Q
X-Ray Structure of Protein from Mus Musculus MM.29898
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.96802, 0.96411 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.541, 73.541, 236.078 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.270 - 2.320 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.834 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.061 | 0.446 |
Number of reflections | 17029 | |
<I/σ(I)> | 16.785 | 5.272 |
Completeness [%] | 98.5 | 100 |
Redundancy | 13.2 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 277 | 10 MG/ML PROTEIN, 18% MEPEG 2K, 0.100 M SODIUM CITRATE, 0.100 M SODIUM ACETATE, vapor diffusion, hanging drop, temperature 277K, pH 5.00 | |
1 | 5 | 277 | 10 MG/ML PROTEIN, 18% MEPEG 2K, 0.100 M SODIUM CITRATE, 0.100 M SODIUM ACETATE, vapor diffusion, hanging drop, temperature 277K, pH 5.00 |