2A3P

Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome with bound molybdate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	173
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.160, 43.700, 57.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.130 - 2.300
R-factor	0.197
Rwork	0.191
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a3m
RMSD bond length	0.012
RMSD bond angle	1.856
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.097	0.050	0.523
Number of reflections	5706
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG 4000, 5% ethylene glycol, 0.2M ZnCl2, 0.1 M imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K