2A3M

Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome (oxidized form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	43.670, 43.876, 57.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.960 - 1.500
R-factor	0.177
Rwork	0.175
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cth
RMSD bond length	0.012
RMSD bond angle	1.218
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.060	0.036	0.238
Number of reflections	18098
Completeness [%]	98.0
Redundancy	6.4	6.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	20% PEG 4000, 5% ethylene glycol, 0.2M ZnCl2, 0.1 M imidazole, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K