2A3L
X-Ray Structure of Adenosine 5'-Monophosphate Deaminase from Arabidopsis Thaliana in Complex with Coformycin 5'-Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 131.325, 131.325, 208.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.910 - 3.340 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RABBIT AMPD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.060 | 0.489 |
| Number of reflections | 16001 | |
| <I/σ(I)> | 22.579 | 3.922 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 295 | 8 MG/ML PROTEIN, 0.40 M MONOAMMONIUM DIHYDROGEN PHOSPHATE, 0.10 M TRI-SODIUM CITRATE, 10% (V/V) ETHANOL, vapor diffusion, hanging drop, temperature 295K, pH 5.60 |
