2A33
X-Ray Structure of a Lysine Decarboxylase-Like Protein from Arabidopsis Thaliana Gene AT2G37210
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-13 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.403, 66.773, 98.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.984 - 1.950 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ydh |
RMSD bond length | 0.021 |
RMSD bond angle | 1.712 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.100 | 0.518 |
Number of reflections | 26424 | |
<I/σ(I)> | 15.009 | 1.756 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 10.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 296 | 10 MG/ML PROTEIN, 22% PEG 2K, 0.084 M MAGNESIUM SULFATE, 0.100 M BISTRIS, vapor diffusion, hanging drop, temperature 296K, pH 6.50 |