2A2M

CRYSTAL STRUCTURE OF a putativeTenA family transcriptional regulator (BT_3146) FROM BACTEROIDES THETAIOTAOMICRON VPI-5482 AT 1.88 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-06
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	F 4 3 2
Unit cell lengths	283.292, 283.292, 283.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	164.400 - 1.880
Rwork	0.142
R-free	0.16500
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.318
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	164.400		1.950
High resolution limit [Å]	1.880	4.040	1.880
Rmerge	0.086	0.819	0.819
Number of reflections	70979	14948	9433
<I/σ(I)>	13.74	33.89	2.04
Completeness [%]	91.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	5	273	1.0M LiCl, 10.0% PEG-6000, 0.1M Citrate pH 5.0 VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K