2A2L
Crystal structure of Klebsiella pneumoniae protein ORFY, Pfam DUF336
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 87 |
| Detector technology | CCD |
| Collection date | 2005-03-07 |
| Detector | ADSC QUANTAM Q315 |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 80.490, 80.490, 207.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.690 - 2.200 |
| R-factor | 0.23725 |
| Rwork | 0.235 |
| R-free | 0.30788 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.397 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.122 | 0.191 |
| Number of reflections | 32826 | |
| <I/σ(I)> | 6.7 | 2.1 |
| Completeness [%] | 92.3 | 79.6 |
| Redundancy | 9.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 30% MPD, 0.1M HEPES, 0.2M Sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
