2A2D
X-ray structure of human n-acetyl galactosamine kinase complexed with Mn-AMPPNP and n-acetyl glactosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-29 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 119.100, 119.100, 65.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.207 |
Rwork | 0.203 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a2c |
RMSD bond length | 0.013 |
RMSD bond angle | 2.490 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 26919 | |
<I/σ(I)> | 15.9 | 3.5 |
Completeness [%] | 98.9 | 98.4 |
Redundancy | 8.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG-3400, Mn-AMPPNP, NaCl, MES, n-acetyl galactosamine, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |