2A28
Atomic-resolution crystal structure of the second SH3 domain of yeast Bzz1 determined from a pseudomerohedrally twinned crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8126 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.840, 51.740, 59.940 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.070 |
| R-factor | 0.1121 |
| Rwork | 0.111 |
| R-free | 0.16630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.100 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Number of reflections | 84816 | |
| <I/σ(I)> | 16.2 | 4.1 |
| Completeness [%] | 96.0 | 93.1 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
