2A26

Crystal structure of the N-terminal, dimerization domain of Siah Interacting Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.826533
Spacegroup name	C 2 2 21
Unit cell lengths	58.793, 152.609, 36.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.200
R-factor	0.17489
Rwork	0.174
R-free	0.19833
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.936
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.046	0.381
Number of reflections	50772
<I/σ(I)>	65	2
Completeness [%]	96.7
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	ammonium sulphate, CAPS, lithium sulphate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	ammonium sulphate, CAPS, lithium sulphate, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K