2A22

Structure of Vacuolar Protein Sorting 29 from Cryptosporidium Parvum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-06
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 41 21 2
Unit cell lengths	78.841, 78.841, 151.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 - 2.198
R-factor	0.17024
Rwork	0.168
R-free	0.21391
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w24
RMSD bond length	0.015
RMSD bond angle	1.490
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	2.280
High resolution limit [Å]	2.198	2.198
Rmerge	0.112
Number of reflections	24958
<I/σ(I)>	22.89	4.45
Completeness [%]	99.7	99.5
Redundancy	7.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	22% w/v PEG 400, 100 mM sodium citrate pH 5.6, 200 mM ammonium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K