2A1W
Anti-cocaine antibody 7.5.21, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.601, 58.395, 98.098 |
| Unit cell angles | 90.00, 110.66, 90.00 |
Refinement procedure
| Resolution | 30.500 - 2.700 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.174 | |
| Number of reflections | 26324 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 99.9 | 99.96 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 16% PEG8000, 0.3M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
