2A1M
Crystal structure of ferrous dioxygen complex of wild-type cytochrome P450cam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-11 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.060, 62.290, 95.640 |
Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
Resolution | 47.820 - 2.100 |
Rwork | 0.191 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dz4 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | BEAST |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.820 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 41808 | |
<I/σ(I)> | 10.5 | 1.9 |
Completeness [%] | 90.2 | 83.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 288 | PEG 4000, potassium chloride, Tris-HCl, D-camphor, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 288K |