2A1K
RB69 single-stranded DNA binding protein core domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 67.700, 67.700, 123.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.870 - 2.000 |
| R-factor | 0.243 |
| Rwork | 0.236 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (7.1SSI) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.870 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.344 |
| Number of reflections | 49458 | |
| <I/σ(I)> | 5 | 1.3 |
| Completeness [%] | 96.3 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG200, MES, magnesium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
