2A1B
Carboxysome shell protein ccmK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.744, 179.987, 69.769 |
| Unit cell angles | 90.00, 119.98, 90.00 |
Refinement procedure
| Resolution | 89.990 - 2.900 |
| R-factor | 0.313 |
| Rwork | 0.313 |
| R-free | 0.34600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a10 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 31983 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 95.7 | 83.5 |
| Redundancy | 4.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | ammonium sulfate, methylpentanediol, dioxane, MES, 1,6 hexanediol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
