2A15
X-ray Crystal Structure of RV0760 from Mycobacterium Tuberculosis at 1.68 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 55.600, 55.600, 104.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.170 - 1.680 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | the model was obtained from a SAD solution from the data collected on SeMet variant of the same protein. |
RMSD bond length | 0.015 |
RMSD bond angle | 1.571 |
Data reduction software | ELVES |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.170 | 1.740 |
High resolution limit [Å] | 1.680 | 1.680 |
Number of reflections | 21399 | |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 1.6 M Na, K Phosphate, 100mM Hepes buffer pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |