2A11

Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793, 1.000, 1.100
Spacegroup name	P 43 21 2
Unit cell lengths	72.589, 72.589, 96.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.286
Rwork	0.259
R-free	0.28570
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.049	0.252
Number of reflections	15508
<I/σ(I)>	28	5
Completeness [%]	99.1	99.6
Redundancy	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	15% PEG 3000, 100 mM Ca-acetate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	15% PEG 3000, 100 mM Ca-acetate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	15% PEG 3000, 100 mM Ca-acetate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K