2A10

carboxysome shell protein ccmK4

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795,0.979,0.9718
Spacegroup name	P 21 21 21
Unit cell lengths	84.758, 87.000, 96.512
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.550 - 1.803
R-factor	0.19436
Rwork	0.193
R-free	0.22708
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.241
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.220	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	66043
<I/σ(I)>	25
Completeness [%]	99.4	100
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	25% ethylene glycol, 20 mM Tris, 100mM NaCl, 10 mM EDTA, 5mM DTT, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K