2A0N

Crystal structure of Imidazole glycerol phosphate synthase subunit hisF (EC 4.1.3.-) (tm1036) from Thermotoga maritima at 1.64 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-31
Detector	ADSC QUANTUM 315
Spacegroup name	P 61 2 2
Unit cell lengths	96.610, 96.610, 155.828
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.310 - 1.640
Rwork	0.167
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vh7
RMSD bond length	0.017
RMSD bond angle	1.908
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.310	48.310	1.680
High resolution limit [Å]	1.640	7.330	1.640
Rmerge	0.096	0.014	0.014
Number of reflections	53271
<I/σ(I)>	6.2	12.5	0.5
Completeness [%]	100.0
Redundancy	12.9	16.9	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	6.9	273	0.2None Nal, 20.0% PEG-3350, No Buffer, pH 6.9, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 273K