2A0N
Crystal structure of Imidazole glycerol phosphate synthase subunit hisF (EC 4.1.3.-) (tm1036) from Thermotoga maritima at 1.64 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-31 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 96.610, 96.610, 155.828 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.310 - 1.640 |
Rwork | 0.167 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vh7 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.908 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.310 | 48.310 | 1.680 |
High resolution limit [Å] | 1.640 | 7.330 | 1.640 |
Rmerge | 0.096 | 0.014 | 0.014 |
Number of reflections | 53271 | ||
<I/σ(I)> | 6.2 | 12.5 | 0.5 |
Completeness [%] | 100.0 | ||
Redundancy | 12.9 | 16.9 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 6.9 | 273 | 0.2None Nal, 20.0% PEG-3350, No Buffer, pH 6.9, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 273K |