2A0M

Arginase superfamily protein from Trypanosoma cruzi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	H 3
Unit cell lengths	128.875, 128.875, 42.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.873 - 1.603
Rwork	0.168
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	low resolution SAD structure
RMSD bond length	0.013
RMSD bond angle	1.343
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005 24/04/2001)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.067	0.047	0.537
Number of reflections	32939
<I/σ(I)>	10.952	23.582	0.997
Completeness [%]	94.6
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	0.4 ul protein 8.7 mg/ml, 0.4 ul crystallization buffer, 100mM potassium bromide, 42% PEG 1000, 100mM Na citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K