2A0L

Crystal structure of KvAP-33H1 Fv complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 42 2 2
Unit cell lengths	136.362, 136.362, 191.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.890 - 3.900
R-factor	0.358
Rwork	0.358
R-free	0.39200
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.700	4.100
High resolution limit [Å]	3.900	3.900
Number of reflections	16257
<I/σ(I)>	9.7	3.1
Completeness [%]		97.6
Redundancy	6.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	sodium citrate, Tric-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	sodium citrate, Tric-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	sodium citrate, Tric-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K