2A0I
F Factor TraI Relaxase Domain bound to F oriT Single-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-31 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 62 |
Unit cell lengths | 77.515, 77.515, 114.369 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.870 - 2.720 |
R-factor | 0.221 |
Rwork | 0.215 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1P4D Chain A |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.820 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.048 | 0.331 |
Number of reflections | 9891 | |
Completeness [%] | 87.1 | 62.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 1000, Imidazole, Calcium Chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 1000 | ||
2 | 1 | 1 | Imidazole | ||
3 | 1 | 1 | Calcium Chloride | ||
4 | 1 | 2 | PEG 1000 | ||
5 | 1 | 2 | Imidazole | ||
6 | 1 | 2 | Calcium Chloride |