2A0B

HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	30.456, 34.924, 110.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.570
Rwork	0.190
R-free	0.24500
RMSD bond length	0.024
RMSD bond angle	0.036
Data reduction software	PROCESS
Data scaling software	PROCESS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.700
High resolution limit [Å]	1.570	1.570
Rmerge	0.049	0.190
Total number of observations	98202 *
Number of reflections	16338
<I/σ(I)>	66
Completeness [%]	95.0 *	89
Redundancy	6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.1	277 *	PROTEIN WAS CRYSTALLIZED FROM 12.5% PEGMME 550, 5 MM ZNSO4, 50 MM ACETIC ACID/SODIUM ACETATE BUFFER, PH 4.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10.6 (mg/ml)
2	1	drop	acetic acid/sodium acetate	50 (mM)
3	1	drop		5 (mM)
4	1	drop	mPEG550	12.5 (%)
5	1	reservoir	mPEG550	13 (%)