2A08
Structure of the yeast YHH6 SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 64 |
| Unit cell lengths | 66.830, 66.830, 52.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.410 - 1.540 |
| R-factor | 0.17329 |
| Rwork | 0.171 |
| R-free | 0.21547 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1OOT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.454 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 19553 | |
| <I/σ(I)> | 15.5 | 4.6 |
| Completeness [%] | 98.1 | 93.6 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.4 M ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
