2A08
Structure of the yeast YHH6 SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9537 |
Spacegroup name | P 64 |
Unit cell lengths | 66.830, 66.830, 52.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.410 - 1.540 |
R-factor | 0.17329 |
Rwork | 0.171 |
R-free | 0.21547 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1OOT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.454 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.600 |
High resolution limit [Å] | 1.540 | 1.540 |
Number of reflections | 19553 | |
<I/σ(I)> | 15.5 | 4.6 |
Completeness [%] | 98.1 | 93.6 |
Redundancy | 4.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.4 M ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |