2A06

Bovine cytochrome bc1 complex with stigmatellin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	128.252, 168.800, 230.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.870 - 2.100
R-factor	0.224
Rwork	0.222
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT USING NATIVE BOVINE AND STIGMATELLIN-BOUND CHICKEN STRUCTURES
Starting model (for MR)	PDB ENTRIES 1BE3 2bcc
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.030
High resolution limit [Å]	1.990	1.990
Number of reflections	305544
<I/σ(I)>	6.1	0.684
Completeness [%]	89.7	51.9
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.65	277	PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.65, VAPOR DIFFUSION, SITTING DROP, temperature 277K