2ZZX
Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.792, 103.501, 90.760 |
| Unit cell angles | 90.00, 101.86, 90.00 |
Refinement procedure
| Resolution | 45.290 - 1.750 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zzv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 133774 | |
| <I/σ(I)> | 18.9 | 3.3 |
| Completeness [%] | 90.1 | 71 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M Potassium phosphate monobasic, 20% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
