2ZZJ
Crystal structure of endo-beta-1,4-glucuronan lyase from fungus Trichoderma reesei
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000, 0.97877, 0.97926, 0.96403 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.287, 62.301, 129.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.940 - 1.800 |
| R-factor | 0.18563 |
| Rwork | 0.184 |
| R-free | 0.22346 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 27647 | |
| <I/σ(I)> | 30.8 | 4 |
| Completeness [%] | 98.5 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% PEG3350, 5.0mM CaCl2, 0.2M ammonium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
