2ZZ8

Crystal structure of LipL32, the most abundant surface protein of pathogenic leptospira spp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	125.895, 125.895, 95.991
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.880 - 2.010
R-factor	0.1831
Rwork	0.181
R-free	0.21630
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.392
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.078	0.720
Number of reflections	59453
<I/σ(I)>	41.9	2.4
Completeness [%]	100.0	100
Redundancy	21.4	20.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	294	2.0M sodium malonate, 0.1M sodium cacodylate, 4%(v/v) butyrolactone, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	6.2	294	2.0M sodium malonate, 0.1M sodium cacodylate, 4%(v/v) butyrolactone, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	6.2	294	2.0M sodium malonate, 0.1M sodium cacodylate, 4%(v/v) butyrolactone, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K