2ZX0
Rhamnose-binding lectin CSL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.040, 74.602, 94.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.19258 |
| Rwork | 0.191 |
| R-free | 0.22976 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 30454 | |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 96.5 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | 15% PEG8000, 50mM potassium phosphate, pH4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | 15% PEG8000, 50mM potassium phosphate, pH4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
