2ZWN

Crystal structure of the novel two-domain type laccase from a metagenome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-26
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	74.669, 100.945, 124.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.010 - 1.700
R-factor	0.16054
Rwork	0.159
R-free	0.18322
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.145
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.010
High resolution limit [Å]	1.700
Number of reflections	98106

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2M Potassium chloride, 0.05M HEPES, pH7.5, 35% Pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, SITTING DROP, temperature 293K