2ZWK
Crystal structure of intimin-Tir90 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2008-09-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 96.780, 96.780, 644.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.670 - 3.100 |
| Rwork | 0.301 |
| R-free | 0.36000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zqk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.670 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.125 | 0.413 |
| Number of reflections | 26817 | |
| <I/σ(I)> | 11.2 | 2.7 |
| Completeness [%] | 93.4 | 95.3 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 2M (NH4)2SO4, 5% 2-Propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
