2ZWB

Neutron crystal structure of wild type human lysozyme in D2O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	NUCLEAR REACTOR
Source details	OTHER
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-07-01
Detector	JAERI BIX-3
Wavelength(s)	2.88
Spacegroup name	P 21 21 21
Unit cell lengths	33.825, 56.883, 60.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.223
Rwork	0.223
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fe0
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.211	0.491
Number of reflections	25514
<I/σ(I)>	3.894	1.11
Completeness [%]	69.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.3	293	PH 4.30(PD), SMALL TUBES, temperature 293K