2ZW7
Crystal structure of bleomycin N-acetyltransferase complexed with bleomycin A2 and coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.050, 70.050, 534.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zw4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.119 | 0.444 |
| Number of reflections | 19469 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 93.6 | 96.5 |
| Redundancy | 5.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 4.0M sodium formate, 0.1M Hepes , pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
