2ZW4
Crystal structure of bleomycin N-acetyltransferase complexed with coenzyme A in the orthorhombic crystal
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924, 0.97942, 0.96189 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.220, 96.330, 181.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.111 | 0.407 |
| Number of reflections | 33968 | |
| <I/σ(I)> | 14.6 | 4.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.0% PEG 400, 1.33M ammonium sulfate, 0.1M Hepes , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
