2ZVZ
Structure of the periplasmic domain of MotB from Salmonella (crystal form III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2008-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.200, 46.240, 173.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.400 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zov |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.220 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 14884 | |
| <I/σ(I)> | 17 | 5.4 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 22% PEG MME 2000, 0.1M Acetate, 0.1M MgSO4, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
