2ZUW

Crystal structure of Galacto-N-biose/Lacto-N-biose I phosphorylase in complex with GlcNAc and sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-03-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	67.905, 112.039, 118.662
Unit cell angles	105.21, 90.43, 107.32

Refinement procedure

Resolution	41.070 - 2.110
R-factor	0.17184
Rwork	0.169
R-free	0.22479
Structure solution method	MAD
RMSD bond length	0.036
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.089	0.327
Number of reflections	180455
<I/σ(I)>	15.936	2.8
Completeness [%]	98.2	97.1
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	277	Na cacodyrate, Mg(NO3)2, PEG 4000, GlcNAc, MgSO4, pH 6.5, VAPOR DIFFUSION, temperature 277K
1		6.5	277	Na cacodyrate, Mg(NO3)2, PEG 4000, GlcNAc, MgSO4, pH 6.5, VAPOR DIFFUSION, temperature 277K