2ZUH

Crystal Structure of Camphor-soaked Ferric Cytochrome P450cam Mutant (D297A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	63.749, 63.749, 250.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.540 - 1.550
R-factor	0.1617
Rwork	0.160
R-free	0.18471
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zax
RMSD bond length	0.011
RMSD bond angle	1.488
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (9.4.09)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.540	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	75680
<I/σ(I)>	12.1
Completeness [%]	99.1	99.9
Redundancy	7.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	12-15% PEG 4000, 50mM Tris-HCl, 250mM KCl, 0.1mM d-Camphor, 10mM dithioerythritol(DTE), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K