2ZTS
Crystal structure of KaiC-like protein PH0186 from hyperthermophilic archaea Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-27 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97905, 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.655, 51.807, 97.468 |
Unit cell angles | 90.00, 122.83, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.070 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.25000 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.870 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.09) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.083 | 0.050 | 0.509 |
Number of reflections | 46906 | ||
<I/σ(I)> | 26.462 | ||
Completeness [%] | 95.5 | 99.8 | 73.4 |
Redundancy | 6.8 | 7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 9% ethanol, 100mM imidazole pH7.6, 200mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |