2ZTK
Crystal structure of homocitrate synthase from Thermus thermophilus complexed with homocitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 135.508, 135.508, 127.062 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.360 - 1.960 |
| R-factor | 0.19442 |
| Rwork | 0.193 |
| R-free | 0.22177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ztj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.552 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.073 | 0.495 |
| Number of reflections | 48641 | |
| <I/σ(I)> | 53.7 | 3.5 |
| Completeness [%] | 99.6 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
