2ZRT
Crystal structure of Zn2+-bound form of des3-23ALG-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28192 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.806, 147.537, 230.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 115.470 - 3.300 |
| R-factor | 0.17411 |
| Rwork | 0.169 |
| R-free | 0.22077 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hqv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.470 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.102 | 0.343 |
| Number of reflections | 27970 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 8.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 15% ethanol, 200mM zinc acetate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
