2ZQP

Crystal Structure of SecYE translocon from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 43 2 2
Unit cell lengths	91.876, 91.876, 240.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.920 - 6.000
R-factor	0.40884
Rwork	0.407
R-free	0.44504
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	the SecYE structure produced by MD simulation using 2ZJS structure
RMSD bond length	0.011
RMSD bond angle	1.420
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	6.000
Number of reflections	2743
Completeness [%]	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	28.5% PEG 3350, 0.18M Na-citrate, 0.015% octaethylene glycol monododecyl ether, 5% glycerol, 0.0001M Pefabloc, VAPOR DIFFUSION, SITTING DROP, temperature 293K