2ZQ3

The crystal structure of the orthorhombic form of hen egg white lysozyme at 1.6 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.542, 67.492, 30.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.450 - 1.600
R-factor	0.192
Rwork	0.192
R-free	0.20700
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.500
High resolution limit [Å]	1.600
Rmerge	0.053
Number of reflections	15914
<I/σ(I)>	48.7
Completeness [%]	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			277	277K