2ZQ0
Crystal structure of SusB complexed with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.717, 112.344, 102.467 |
| Unit cell angles | 90.00, 100.61, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.171 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d73 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.328 |
| Number of reflections | 217504 | |
| <I/σ(I)> | 49.9 | 7.9 |
| Completeness [%] | 98.2 | 95.8 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 6-12% PEG 6000, 0.1M Imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
