2ZOK
Crystal structure of H-2Db in complex with JHMV epitope S510
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.538, 86.056, 152.072 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 56.980 - 2.100 |
| R-factor | 0.20936 |
| Rwork | 0.206 |
| R-free | 0.26614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bz9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.741 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.400 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 | 0.185 |
| Number of reflections | 113937 | |
| <I/σ(I)> | 8.2 | 4.8 |
| Completeness [%] | 95.4 | 97.3 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M sodium citrate, 28% PEG 3350, 0.2M lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
