2ZOF
Crystal structure of mouse carnosinase CN2 complexed with MN and bestatin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.8926, 1.8941, 1.7926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.496, 199.181, 55.215 |
| Unit cell angles | 90.00, 118.92, 90.00 |
Refinement procedure
| Resolution | 43.030 - 2.300 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23900 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.424 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 | 0.235 |
| Number of reflections | 39799 | |
| Completeness [%] | 86.8 | 30.9 |
| Redundancy | 6.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 20% PEG 3350, 20% GLYCEROL, 0.2M HF, 25MH TRIS-HCL, 50MM NACL, 0.2MM MNCL2, 1MM DTT, 30MM BESTATIN, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
