2ZOA
Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) COLLECTED AT 1.280 ANGSTROM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2804 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 164.343, 164.343, 164.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.500 - 2.400 |
| R-factor | 0.15953 |
| Rwork | 0.159 |
| R-free | 0.17893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dxn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.607 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 57620 | |
| <I/σ(I)> | 23.1 | 2.4 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 5.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
