2ZO7

Crystal Structure of a Kusabira-Cyan Mutant (KCY-R1), a Cyan/Green-Emitting GFP-Like Protein

Replaces:  2EJI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	96.689, 45.016, 51.158
Unit cell angles	90.00, 94.98, 90.00

Refinement procedure

Resolution	18.700 - 1.580
R-factor	0.178
Rwork	0.178
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zo6
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.580	1.580
Rmerge	0.060	0.257
Number of reflections	29798
<I/σ(I)>		5
Completeness [%]	98.0	91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	35-40% PEG 8000, 0.2M (NH4)2SO4, 0.1M SODIUM CACODYLATE, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 291K