2ZO2
Mouse NP95 SRA domain non-specific DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.551, 81.551, 182.165 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.670 - 3.090 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zo0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.670 | 3.200 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.111 | 0.392 |
| Number of reflections | 6782 | |
| <I/σ(I)> | 13.6 | 4.5 |
| Completeness [%] | 94.3 | 93.7 |
| Redundancy | 7.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% (v/v) polyethylene glycol 3350 or 10000, 0.2-0.4M NaCl, 0.1M MES(pH 5.8-6.2), VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | PEG 10000 | ||
| 3 | 1 | 1 | NaCL | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | PEG 10000 | ||
| 7 | 1 | 2 | NaCL | ||
| 8 | 1 | 2 | MES |
