2ZO1
Mouse NP95 SRA domain DNA specific complex 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.026, 62.026, 164.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.230 - 1.960 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zo0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.230 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.076 | 0.465 |
| Number of reflections | 23766 | |
| <I/σ(I)> | 17.4 | 2.6 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 20% (v/v) polyethylene glycol 3350, 0.4M NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | NaCL | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | NaCL | ||
| 6 | 1 | 2 | H2O |
