2ZM1
Crystal structure of imidazo pyrazin 1 bound to the kinase domain of human LCK, (auto-phosphorylated on TYR394)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-02-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.308, 73.830, 92.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.17209 |
| Rwork | 0.165 |
| R-free | 0.23177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lck |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.605 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.440 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.087 | 0.190 |
| Number of reflections | 17341 | |
| <I/σ(I)> | 13.8 | 7.9 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M (NH4)2SO4, 0.1M Sodium Cacodylate, 30% PEG8000, 5.2% MPD, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
